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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
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- Product number
- PB9104 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cyclin B1/CCNB1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CCNB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human. CCNB information: Molecular Weight: 48337 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells.
Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways.
PKMYT1 is associated with prostate cancer malignancy and may serve as a therapeutic target.
IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma.
Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells.
Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells.
Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells.
Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide.
XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line.
Suppression of C-myc expression associates with anti-proliferation of aloe-emodin on gastric cancer cells.
Leng X, Kan H, Wu Q, Li C, Zheng Y, Peng G
Molecules (Basel, Switzerland) 2022 Feb 27;27(5)
Molecules (Basel, Switzerland) 2022 Feb 27;27(5)
Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways.
Liu H, Mao Y, Xia B, Long C, Kuang X, Huang H, Ning J, Ma X, Zhang H, Wang R, Tang H, Du H, Yan J, Zhang Q, Zhang X, Shen H
BioMed research international 2020;2020:6061894
BioMed research international 2020;2020:6061894
PKMYT1 is associated with prostate cancer malignancy and may serve as a therapeutic target.
Wang J, Wang L, Chen S, Peng H, Xiao L, E Du, Liu Y, Lin D, Wang Y, Xu Y, Yang K
Gene 2020 Jun 20;744:144608
Gene 2020 Jun 20;744:144608
IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma.
Wang L, Bi XW, Zhu YJ, He YZ, Lai QY, Xia ZJ, Cai QQ
Cancer communications (London, England) 2018 Oct 19;38(1):62
Cancer communications (London, England) 2018 Oct 19;38(1):62
Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells.
Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J
International journal of clinical and experimental pathology 2014;7(9):5750-61
International journal of clinical and experimental pathology 2014;7(9):5750-61
Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells.
Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G
Molecular medicine reports 2014 Jul;10(1):89-94
Molecular medicine reports 2014 Jul;10(1):89-94
Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells.
Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X
Molecular biology reports 2011 Mar;38(3):1741-7
Molecular biology reports 2011 Mar;38(3):1741-7
Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide.
Ling H, Wen L, Ji XX, Tang YL, He J, Tan H, Xia H, Zhou JG, Su Q
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2010 Mar;43(3):271-8
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2010 Mar;43(3):271-8
XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line.
Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y
Chemico-biological interactions 2010 Jan 5;183(1):133-41
Chemico-biological interactions 2010 Jan 5;183(1):133-41
Suppression of C-myc expression associates with anti-proliferation of aloe-emodin on gastric cancer cells.
Guo J, Xiao B, Liu Q, Gong Z, Le Y
Cancer investigation 2008 May;26(4):369-74
Cancer investigation 2008 May;26(4):369-74
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclin B1 using anti-Cyclin B1 antibody (PB9104). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human cyclin b1 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin B1 antigen affinity purified polyclonal antibody (Catalog # PB9104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin B1 at approximately 39KD. The expected band size for Cyclin B1 is at 39KD.
- Additional image
