PA5-36712

antibody from Invitrogen Antibodies

Targeting: CCNB1 CCNB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36712

Provider

Invitrogen Antibodies

Product name

Phospho-Cyclin B1 (Ser126) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 60 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Cyclin B1 (Ser126) in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: HEK293T whole cell lysate (40 µg), Lane 3: SGC7901 whole cell lysate (40 µg), Lane 4: 3T3-L1 whole cell lysate (40 µg), Lane 5: PC12 whole cell lysate (40 µg). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Cyclin B1 pSer126 using Phospho-Cyclin B1 pSer126 polyclonal antibody (Product # PA5-36712) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with EGF (0.1 ng/mL, 30 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-Cyclin B1 (Ser126) in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-Cyclin B1 (Ser126) in paraffin-embedded human lung carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-Cyclin B1 (Ser126) in paraffin-embedded human breast carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.