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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-36712 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Cyclin B1 (Ser126) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 60 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Cyclin B1 (Ser126) in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: HEK293T whole cell lysate (40 µg), Lane 3: SGC7901 whole cell lysate (40 µg), Lane 4: 3T3-L1 whole cell lysate (40 µg), Lane 5: PC12 whole cell lysate (40 µg). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Cyclin B1 pSer126 using Phospho-Cyclin B1 pSer126 polyclonal antibody (Product # PA5-36712) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with EGF (0.1 ng/mL, 30 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Cyclin B1 (Ser126) in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Cyclin B1 (Ser126) in paraffin-embedded human lung carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Cyclin B1 (Ser126) in paraffin-embedded human breast carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cyclin B1 (Ser126) polyclonal antibody (Product # PA5-36712) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
