TA319230

antibody from OriGene

Targeting: CCNB1 CCNB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319230

Provider

OriGene

Product name

Rabbit polyclonal Cyclin B1 phospho S126 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal Cyclin B1 phospho S126 antibody

Reactivity

Canine

Host

Rabbit

Conjugate

Unconjugated

Epitope

CCNB1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

2.01 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB using Anti-Cyclin B pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B(arrowheads) in various whole cell lysates.? Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). The primary antibody was used at 1:100. HRP conjugated Gt-a-Rabbit IgG [H&L] was used at 1:5000.

Validation comment

WB