orb385903

antibody from Biorbyt

Targeting: CCNB1 CCNB

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

orb385903

Provider

Biorbyt

Product name

Cyclin B1 Antibody / CCNB1

Antibody type

Monoclonal

Description

Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the CCNB1-Cdk1 complex is activated, it is not deactivated. CCNB1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. Once activated, CCNB1-Cdk1 promotes several of the events of early mitosis. The active complex phosphorylates and activates 13S condensin, which helps to condense chromosomes. Another important function of the complex is to break down the nuclear envelope. The nuclear envelope is a membranous structure containing large protein complexes supported by a network of nuclear lamins. Phosphorylation of the lamins by CCBN1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. The destruction of the nuclear envelope is important because it allows the mitotic spindle to access the chromosomes. [Wiki]

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

BCLB1-1

Vial size

20 ug

Storage

Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).