Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB18121 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18121, RRID:AB_10713030
- Product name
- CCNB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CCNB1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx.
Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites.
Walsh S, Margolis SS, Kornbluth S
Molecular cancer research : MCR 2003 Feb;1(4):280-9
Molecular cancer research : MCR 2003 Feb;1(4):280-9
Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites.
Yang J, Song H, Walsh S, Bardes ES, Kornbluth S
The Journal of biological chemistry 2001 Feb 2;276(5):3604-9
The Journal of biological chemistry 2001 Feb 2;276(5):3604-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells, treated with serum (10 %, 15 min), using CCNB1 polyclonal antibody (Cat # PAB18121).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells, treated with Forskolin (40 nM, 30 mins), using CCNB1 polyclonal antibody (Cat # PAB18121).Peptide "+" means "peptide blocking".
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CCNB1 polyclonal antibody (Cat # PAB18121).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)