Antibody data
- Antibody Data
- Antigen structure
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- Product number
- AMM00437G - Provider product page
- Provider
- Leading Biology
- Product name
- Cyclin B1 (G2- & M-phase Cyclin) Antibody - Without BSA and Azide
- Antibody type
- Monoclonal
- Description
- It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
- Reactivity
- Human, Mouse, Hamster
- Host
- Mouse
- Antibody clone number
- V92.1
- Storage
- Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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