R1566P

antibody from Acris Antibodies GmbH

Targeting: CCNB1 CCNB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1566P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1566P, RRID:AB_979183

Product name

anti Cyclin B1 pSer126

Antibody type

Polyclonal

Antigen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 120-131 of Human Cyclin B1 protein.

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

0.6 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot using Affinity Purified anti- Cyclin B1pS126 antibody shows detection of a band ~48 kDa corresponding tophosphorylated human Cyclin B1 (arrowheads) in various whole celllysates. Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 -H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G(Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lanecontains approximately 50 µg of lysates, separated by 12% SDS-PAGEusing a 5% stack run at 100 volts until the dye front cleared the bottom ofthe gel. Transfer occurred overnight at 4° C at 15 mAmps. Themembrane was blocked with 5% non-fat dry milk in TTBS for 1 h at roomtemperature followed by addition of a 1:100 dilution of the antibodyallowed to react for 2h at room temperature. After washes with TTBS a1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (611-103-122) was added for 1 h at room temperature. After additional washes themembrane was incubated with ECL mix 1:1 for ~3 min. Excess detectionsolution was drained off and the membrane was exposed to Kodak filmX-omat blue XB-1 for about 20 sec. Other detection systems will yieldsimilar results. Personnel Communication, Luca Cote, Temple U.