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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX48868 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48868, RRID:AB_11163898
- Product name
- ELK1 (phospho Ser383) antibody
- Antibody type
- Polyclonal
- Host
- Rabbit
- Vial size
- 100 µl
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis shows GeneTex's Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 µg was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1,000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.
- Validation comment
- WB
