Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-4625 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ELK1 (Ser383) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, mouse, non-human primate and zebrafish based on 100% sequence homology. This antibody is specific for ~46 kDa Elk-1 phosphorylated at Ser383 in Western blots using phospho and dephospho Elk-1 recombinant protein. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of dephospho (Lane 1) and phospho (Lane 2) recombinant Elk-1 showing phospho-specific immunolabeling of the ~46 kDa Elk-1 protein. Positive control: recombinant Elk-1 protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of ELK1 in recombinant Elk-1. showing specific immunolabeling of a band at ~46 kDa corresponding to Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by pre-adsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown). Samples were incubated in Phospho-ELK1 (Ser383) polyclonal antibody (Product # pA1-4625).
