MA5-16973

antibody from Invitrogen Antibodies

Targeting: EPCAM 17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-16973

Provider

Invitrogen Antibodies

Product name

EpCAM Monoclonal Antibody (VU-1D9)

Antibody type

Monoclonal

Antigen

Other

Description

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is large bowel. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. This product is a low-endotoxin, preservative-free formulation. Mouse anti Human CD326 antibody, clone VU-1D9 recognizes epithelial cell adhesion molecule (Ep-CAM), a approximately 34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

VU-1D9

Vial size

500 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

New frontiers in circulating tumor cell analysis: A reference guide for biomolecular profiling toward translational clinical use.

Becker TM, Caixeiro NJ, Lim SH, Tognela A, Kienzle N, Scott KF, Spring KJ, de Souza P
International journal of cancer 2014 Jun 1;134(11):2523-33

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Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.

Mosoyan G, Nagi C, Marukian S, Teixeira A, Simonian A, Resnick-Silverman L, DiFeo A, Johnston D, Reynolds SR, Roses DF, Mosoian A
PloS one 2013;8(1):e55145

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Systematic analysis and validation of differential gene expression in ovarian serous adenocarcinomas and normal ovary.

Bauerschlag D, Bräutigam K, Moll R, Sehouli J, Mustea A, Salehin D, Krajewska M, Reed JC, Maass N, Hampton GM, Meinhold-Heerlein I
Journal of cancer research and clinical oncology 2013 Feb;139(2):347-55

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Neuropilin-2 Is upregulated in lung cancer cells during TGF-β1-induced epithelial-mesenchymal transition.

Nasarre P, Gemmill RM, Potiron VA, Roche J, Lu X, Barón AE, Korch C, Garrett-Mayer E, Lagana A, Howe PH, Drabkin HA
Cancer research 2013 Dec 1;73(23):7111-21

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Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells.

Horio M, Sato M, Takeyama Y, Elshazley M, Yamashita R, Hase T, Yoshida K, Usami N, Yokoi K, Sekido Y, Kondo M, Toyokuni S, Gazdar AF, Minna JD, Hasegawa Y
Annals of surgical oncology 2012 Jul;19 Suppl 3(Suppl 3):S634-45

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A pilot study to explore circulating tumour cells in pancreatic cancer as a novel biomarker.

Khoja L, Backen A, Sloane R, Menasce L, Ryder D, Krebs M, Board R, Clack G, Hughes A, Blackhall F, Valle JW, Dive C
British journal of cancer 2012 Jan 31;106(3):508-16

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of EpCAM was performed using HT-29 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EpCAM Mouse Monoclonal Antibody (Product # MA5-16973) at 1:500 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HT-29 cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HT-29 cells, which is a positive model for EpCAM expression showing a plasma membrane localization. Panel e represents the merged image of HeLa cells, that are null for EpCAM protein expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.