NBP2-33051
antibody from Novus Biologicals
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [5]
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Validation data
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- Product number
- NBP2-33051 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal EpCAM/TROP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
Submitted references Acquired Resistance Mutations to ALK Inhibitors Identified by Single Circulating Tumor Cell Sequencing in ALK-Rearranged Non-Small-Cell Lung Cancer.
Establishment and characterization of an oral tongue squamous cell carcinoma cell line from a never-smoking patient.
EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder.
The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
Flow cytometric analysis of antigen expression in malignant and normal renal cells.
Immunohistochemical localization of epithelial glycoprotein EGP-2 and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors.
Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors.
Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors.
Pailler E, Faugeroux V, Oulhen M, Mezquita L, Laporte M, Honoré A, Lecluse Y, Queffelec P, NgoCamus M, Nicotra C, Remon J, Lacroix L, Planchard D, Friboulet L, Besse B, Farace F
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Nov 15;25(22):6671-6682
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Nov 15;25(22):6671-6682
Establishment and characterization of an oral tongue squamous cell carcinoma cell line from a never-smoking patient.
Wang SJ, Asthana S, van Zante A, Heaton CM, Phuchareon J, Stein L, Higuchi S, Kishimoto T, Chiu CY, Olshen AB, McCormick F, Tetsu O
Oral oncology 2017 Jun;69:1-10
Oral oncology 2017 Jun;69:1-10
EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder.
Brunner A, Prelog M, Verdorfer I, Tzankov A, Mikuz G, Ensinger C
Journal of clinical pathology 2008 Mar;61(3):307-10
Journal of clinical pathology 2008 Mar;61(3):307-10
The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
Winter MJ, Nagtegaal ID, van Krieken JH, Litvinov SV
The American journal of pathology 2003 Dec;163(6):2139-48
The American journal of pathology 2003 Dec;163(6):2139-48
Flow cytometric analysis of antigen expression in malignant and normal renal cells.
Li G, Passebosc-Faure K, Lambert C, Gentil-Perret A, Blanc F, Oosterwijk E, Mosnier JF, Genin C, Tostain J
Anticancer research 2000 Jul-Aug;20(4):2773-8
Anticancer research 2000 Jul-Aug;20(4):2773-8
Immunohistochemical localization of epithelial glycoprotein EGP-2 and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors.
Ogura E, Senzaki H, Yoshizawa K, Hioki K, Tsubura A
Anticancer research 1998 Sep-Oct;18(5B):3669-75
Anticancer research 1998 Sep-Oct;18(5B):3669-75
Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors.
Tsubura A, Senzaki H, Sasaki M, Hilgers J, Morii S
Journal of cutaneous pathology 1992 Feb;19(1):73-9
Journal of cutaneous pathology 1992 Feb;19(1):73-9
Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors.
Tsubura A, Senzaki H, Sasaki M, Hilgers J, Morii S
Journal of cutaneous pathology 1992 Feb;19(1):73-9
Journal of cutaneous pathology 1992 Feb;19(1):73-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Ep-CAM monoclonal antibody (VU-1D9).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Analysis using Azide and BSA Free version of NBP2-33051. Surface staining of HT29 cells using Ep-CAM MAb (VU-1D9) (red) and isotype control antibody (green).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) PerCP-CyTM5.5. Image using the PE form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) purified. Image using the PE form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Surface staining of Human Colon HT29 cells using EpCAM/TROP1 Antibody (VU-1D9) (red) and isotype control antibody (green).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM/TROP1 Antibody (VU-1D9).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).