NBP2-33078
antibody from Novus Biologicals
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [5]
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Validation data
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- Product number
- NBP2-33078 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal EpCAM/TROP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (VU-1D9) - Azide and BSA Free [NBP2-33078] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Ep-CAM monoclonal antibody (VU-1D9).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) - Azide and BSA Free [NBP2-33078] - Surface staining of HT29 cells using Ep-CAM MAb (VU-1D9) (red) and isotype control antibody (green).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) - Azide and BSA Free [NBP2-33078] - An intracellular stain was performed on Caco-2 cells with EpCAM/TROP1 Antibody [VU-1D9] NBP2-33078PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) - Azide and BSA Free [NBP2-33078] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM/TROP1 Antibody (VU-1D9).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) - Azide and BSA Free [NBP2-33078] - Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) PerCP-CyTM5.5. Image using the PE form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) - Azide and BSA Free [NBP2-33078] - Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) purified. Image using the PE form of this antibody.