21050-1-AP

antibody from Proteintech Group

Targeting: EPCAM 17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1

Provider product page for 21050-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [33]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 21050-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21050-1-AP, RRID:AB_10693684
Product name: EPCAM antibody
Antibody type: Polyclonal
Description: KD/KO validated EPCAM antibody (Cat. #21050-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

EPCAM protein structure - 21050-1-AP shown in red.

Submitted references Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.

Cao W, Li Y, Sun H, Yang C, Zhu J, Xie C, Li X, Wu J, Geng S, Wang L, Sun L, Geng G, Han H, Zhong C
Frontiers in cell and developmental biology 2021;9:679806

Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy.

Li W, Zhou Y, Wu Z, Shi Y, Tian E, Zhu Y, Wang T, Dou W, Meng X, Chen M, Zhai B, Zhu D
Frontiers in pharmacology 2021;12:724306

Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.

Liu C, Pan Z, Chen Q, Chen Z, Liu W, Wu L, Jiang M, Lin W, Zhang Y, Lin W, Zhou R, Zhao L
Journal of experimental & clinical cancer research : CR 2021 Sep 22;40(1):297

Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.

Gao X, Teng X, Dai Y, Li J
ACS sensors 2021 Oct 22;6(10):3611-3620

The stemness of hepatocytes is maintained by high levels of lipopolysaccharide via YAP1 activation.

Shao C, Yang X, Jing Y, Hou X, Huang Y, Zong C, Gao L, Liu W, Jiang J, Ye F, Shi J, Zhao Q, Li R, Zhang X, Wei L
Stem cell research & therapy 2021 Jun 10;12(1):342

EGFR activation-induced decreases in claudin1 promote MUC5AC expression and exacerbate asthma in mice.

Jia Z, Bao K, Wei P, Yu X, Zhang Y, Wang X, Wang X, Yao L, Li L, Wu P, Yuan W, Wang S, Zheng J, Hua Y, Hong M
Mucosal immunology 2021 Jan;14(1):125-134

A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.

Huh D, Passarelli MC, Gao J, Dusmatova SN, Goin C, Fish L, Pinzaru AM, Molina H, Ren Z, McMillan EA, Asgharian H, Goodarzi H, Tavazoie SF
The EMBO journal 2021 Jan 15;40(2):e106696

Expression of Cancer Stem Cell Markers EpCAM and CD90 Is Correlated with Anti- and Pro-Oncogenic EphA2 Signaling in Hepatocellular Carcinoma.

Asakura N, Nakamura N, Muroi A, Nojima Y, Yamashita T, Kaneko S, Ikeda K, Koshikawa N, Suzuki T
International journal of molecular sciences 2021 Aug 11;22(16)

Near-infrared-driven fluorescent nanomotors for detection of circulating tumor cells in whole blood.

Xu P, Yu Y, Li T, Chen H, Wang Q, Wang M, Wan M, Mao C
Analytica chimica acta 2020 Sep 8;1129:60-68

Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.

Chen Z, Zhou L, Liu L, Hou Y, Xiong M, Yang Y, Hu J, Chen K
Nature communications 2020 Oct 8;11(1):5077

Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.

Yang Y, Li L, Xu C, Wang Y, Wang Z, Chen M, Jiang Z, Pan J, Yang C, Li X, Song K, Yan J, Xie W, Wu X, Chen Z, Yuan Y, Zheng S, Yan J, Huang J, Qiu F
Gut 2020 Oct 29;

MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth.

Li M, Pan M, You C, Zhao F, Wu D, Guo M, Xu H, Shi F, Zheng D, Dou J
Breast cancer research : BCR 2020 Mar 6;22(1):26

Hedgehog signaling promotes sorafenib resistance in hepatocellular carcinoma patient-derived organoids.

Wang S, Wang Y, Xun X, Zhang C, Xiang X, Cheng Q, Hu S, Li Z, Zhu J
Journal of experimental & clinical cancer research : CR 2020 Jan 28;39(1):22

Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier.

Lu H, Jin D, Zhu L, Guo T, Li X, Peng XX, Mo G, Tang L, Zhang GJ, Yang F
Biosensors & bioelectronics 2020 Dec 15;170:112662

miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.

Shi DM, Shi XL, Xing KL, Zhou HX, Lu LL, Wu WZ
Cellular signalling 2020 Aug;72:109650

In Vivo Study on the Effects of Xiaoaiping on the Stemness of Hepatocellular Carcinoma Cells.

Zhan J, Shi LL, Wang Y, Wei B, Yang SL
Evidence-based complementary and alternative medicine : eCAM 2019;2019:4738243

Hepatocyte growth factor activator inhibitor-2 stabilizes Epcam and maintains epithelial organization in the mouse intestine.

Kawaguchi M, Yamamoto K, Takeda N, Fukushima T, Yamashita F, Sato K, Kitamura K, Hippo Y, Janetka JW, Kataoka H
Communications biology 2019;2:11

High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells.

Han S, Wang T, Chen Y, Han Z, Guo L, Wu Z, Yan W, Wei H, Liu T, Zhao J, Zhou W, Yang X, Xiao J
American journal of translational research 2019;11(1):442-452

Genistein inhibits nasopharyngeal cancer stem cells through sonic hedgehog signaling.

Zhang Q, Cao WS, Wang XQ, Zhang M, Lu XM, Chen JQ, Chen Y, Ge MM, Zhong CY, Han HY
Phytotherapy research : PTR 2019 Oct;33(10):2783-2791

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Xie C, Zhu J, Wang X, Chen J, Geng S, Wu J, Zhong C, Li X
Journal of experimental & clinical cancer research : CR 2019 Jan 28;38(1):39

Deglycosylation of epithelial cell adhesion molecule affects epithelial to mesenchymal transition in breast cancer cells.

Liu X, Yang L, Zhang D, Liu T, Yan Q, Yang X
Journal of cellular physiology 2019 Apr;234(4):4504-4514

Curcumin attenuates resistance to irinotecan via induction of apoptosis of cancer stem cells in chemoresistant colon cancer cells.

Su P, Yang Y, Wang G, Chen X, Ju Y
International journal of oncology 2018 Sep;53(3):1343-1353

A simple microdevice for single cell capture, array, release, and fast staining using oscillatory method.

Cheng D, Yu Y, Han C, Cao M, Yang G, Liu J, Chen X, Peng Z
Biomicrofluidics 2018 May;12(3):034105

Tropism of liver epithelial cells toward hepatocellular carcinoma in vitro and in vivo with altering gene expression of cancer stem cells.

Jeng KS, Jeng CJ, Jeng WJ, Sheen IS, Li SY, Lu SJ, Chang CF
American journal of surgery 2018 Apr;215(4):735-743

EpCAM and COX‑2 expression are positively correlated in human breast cancer.

Gao S, Sun Y, Liu X, Zhang D, Yang X
Molecular medicine reports 2017 Jun;15(6):3755-3760

Bufalin suppresses cancer stem-like cells in gemcitabine-resistant pancreatic cancer cells via Hedgehog signaling.

Wang H, Ning Z, Li Y, Zhu X, Meng Z
Molecular medicine reports 2016 Sep;14(3):1907-14

Liver epithelial cells inhibit proliferation and invasiveness of hepatoma cells.

Jeng KS, Jeng CJ, Jeng WJ, Sheen IS, Li SY, Hung ZH, Hsiau HI, Yu MC, Chang CF
Oncology reports 2016 Mar;35(3):1622-8

EpCAM expression in breast cancer cells is associated with enhanced bone metastasis formation.

Hiraga T, Ito S, Nakamura H
International journal of cancer 2016 Apr 1;138(7):1698-708

High LIN28A Expressing Ovarian Cancer Cells Secrete Exosomes That Induce Invasion and Migration in HEK293 Cells.

Enriquez VA, Cleys ER, Da Silveira JC, Spillman MA, Winger QA, Bouma GJ
BioMed research international 2015;2015:701390

ErbB2 Pathway Activation upon Smad4 Loss Promotes Lung Tumor Growth and Metastasis.

Liu J, Cho SN, Akkanti B, Jin N, Mao J, Long W, Chen T, Zhang Y, Tang X, Wistub II, Creighton CJ, Kheradmand F, DeMayo FJ
Cell reports 2015 Mar 10;10(9):1599-1613

Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.

Xu C, Fillmore CM, Koyama S, Wu H, Zhao Y, Chen Z, Herter-Sprie GS, Akbay EA, Tchaicha JH, Altabef A, Reibel JB, Walton Z, Ji H, Watanabe H, Jänne PA, Castrillon DH, Rustgi AK, Bass AJ, Freeman GJ, Padera RF, Dranoff G, Hammerman PS, Kim CF, Wong KK
Cancer cell 2014 May 12;25(5):590-604

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K
Journal of proteomics 2013 Mar 27;80:171-82

Recovery of mature hepatocytic phenotype following bile ductular transdifferentiation of rat hepatocytes in vitro.

Sone M, Nishikawa Y, Nagahama Y, Kumagai E, Doi Y, Omori Y, Yoshioka T, Tokairin T, Yoshida M, Sugiyama T, Enomoto K
The American journal of pathology 2012 Dec;181(6):2094-104

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP(EPCAM antibody) at dilution of 1:200
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The EPCAM antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human EPCAM. This antibody recognizes human, mouse antigen. The EPCAM antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 21050-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 21050-1-AP(EPCAM antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue