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LearnAF960
antibody from Novus Biologicals
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AF960 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal EpCAM/TROP1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system.
HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone.
Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration.
EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells That Promote Epithelial-to-Mesenchymal Transition.
Stromal gene expression defines poor-prognosis subtypes in colorectal cancer.
Garnier D, Li R, Delbos F, Fourrier A, Collet C, Guguen-Guillouzo C, Chesné C, Nguyen TH
Scientific reports 2018 May 29;8(1):8222
Scientific reports 2018 May 29;8(1):8222
HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone.
Day KC, Lorenzatti Hiles G, Kozminsky M, Dawsey SJ, Paul A, Broses LJ, Shah R, Kunja LP, Hall C, Palanisamy N, Daignault-Newton S, El-Sawy L, Wilson SJ, Chou A, Ignatoski KW, Keller E, Thomas D, Nagrath S, Morgan T, Day ML
Cancer research 2017 Jan 1;77(1):74-85
Cancer research 2017 Jan 1;77(1):74-85
Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration.
Ueki T, Yoshihara A, Teramura Y, Takai M
Science and technology of advanced materials 2016;17(1):807-815
Science and technology of advanced materials 2016;17(1):807-815
EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells That Promote Epithelial-to-Mesenchymal Transition.
Hsu YT, Osmulski P, Wang Y, Huang YW, Liu L, Ruan J, Jin VX, Kirma NB, Gaczynska ME, Huang TH
Cancer research 2016 Nov 1;76(21):6171-6182
Cancer research 2016 Nov 1;76(21):6171-6182
Stromal gene expression defines poor-prognosis subtypes in colorectal cancer.
Calon A, Lonardo E, Berenguer-Llergo A, Espinet E, Hernando-Momblona X, Iglesias M, Sevillano M, Palomo-Ponce S, Tauriello DV, Byrom D, Cortina C, Morral C, Barceló C, Tosi S, Riera A, Attolini CS, Rossell D, Sancho E, Batlle E
Nature genetics 2015 Apr;47(4):320-9
Nature genetics 2015 Apr;47(4):320-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human EpCAM/TROP-1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, MCF-7 human breast cancer cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EpCAM/TROP-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- EpCAM/TROP-1 in Human Colon. EpCAM/TROP-1 in Human Colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- EpCAM/TROP-1 in Human Colon. EpCAM/TROP-1 in Human Colon.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of EpCAM/TROP-1 in HT-29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
