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LearnMAB12251
antibody from Abnova Corporation
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB12251 - Provider product page
- Provider
- Abnova Corporation
- Product name
- EPCAM monoclonal antibody, clone 7B5C10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant human EPCAM.
- Isotype
- IgG
- Antibody clone number
- 7B5C10
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human EPCAM (AA: 24-265) recombinant protein (Expected MW is 53.4 kDa) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [EPCAM (AA: 24-265)-hIgGFc transfected HEK293 cells] with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: A431 cell lysate; Lane 2: MCF-7 cell lysate with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human cervical cancer tissues (A) and esophageal cancer tissues (B) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of A431 cells with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:400 dilution (Green) and negative control (Red).
- Validation comment
- Flow Cytometry
