V3463

antibody from NSJ Bioreagents

Targeting: EPCAM 17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3463

Provider

NSJ Bioreagents

Product name

EpCAM Antibody

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

EGP40/1798

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)

Storage

Store the Ep-CAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human MCF7 cell lysate with Ep-CAM antibody (clone EGP40/1798). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colorectal carcinoma and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Ep-CAM antibody (clone EGP40/1798). These results demonstrate the foremost specificity of the EGP40/1798 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free Ep-CAM Antibody (clone EGP40/1798). Confirmation of Integrity and Purity of the Antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Ep-CAM antibody (clone EGP40/1798) as confirmation of integrity and purity.