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LearnV3525HRP
antibody from NSJ Bioreagents
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Protein array [1]
- Other assay [1]
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Validation data
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- Product number
- V3525HRP - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EpCAM Antibody (Extracellular domain)
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Horseradish Peroxidase
- Antibody clone number
- EGP40/1372
- Vial size
- 50 µg
- Concentration
- 0.1 mg/ml
- Storage
- Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human ovarian carcinoma with HRP-conjugated EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Protein array validation of the EpCAM antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using the EpCAM antibody (clone EGP40/1372). These results demonstrate the foremost specificity of the EGP40/1372 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, Unlabeled, BSA-Free EpCAM Antibody (clone EGP40/1372). Confirmation of Integrity and Purity of the Antibody.
