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LearnV3525
antibody from NSJ Bioreagents
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Protein array [1]
- Other assay [1]
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Validation data
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- Product number
- V3525 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Anti-EpCAM Antibody / Extracellular domain
- Antibody type
- Monoclonal
- Description
- This highly specific Anti-EpCAM antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human, dog, cat samples.
- Reactivity
- Human, Canine, Feline
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- EGP40/1372
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEK293, 2) A431 and 3) HCT116 cell lysate with anti-EpCAM antibody (clone EGP40/1372). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human colorectal carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Protein array validation of the anti-EpCAM antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using the anti-EpCAM antibody (clone EGP40/1372). These results demonstrate the foremost specificity of the EGP40/1372 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Anti-EpCAM Antibody (clone EGP40/1372). Confirmation of Integrity and Purity of the Antibody.
