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LearnPA5-29634
antibody from Invitrogen Antibodies
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- PA5-29634 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EpCAM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HCT116, MCF-7, Molt-4.
- Concentration
- 1.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EpCAM/CD326 using 30 µg of THP-1 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an EpCAM/CD326 polyclonal antibody (Product # PA5-29634) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EpCAM/CD326 using 30 µg Molt-4 whole cell extract. Samples were loaded onto a 12% SDS-PAGE gel and probed with an EpCAM/CD326 polyclonal antibody (Product # PA5-29634) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EpCAM Polyclonal Antibody (Product # PA5-29634). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM Polyclonal Antibody (Product # PA5-29634) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EpCAM was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a EpCAM Polyclonal Antibody (Product # PA5-29634) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of EpCAM was performed in paraffin-embedded mouse duodenum tissue using EpCAM Polyclonal Antibody (Product # PA5-29634) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using Ep-CAM (Product # PA5-29634) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
