PA1-26646

antibody from Invitrogen Antibodies

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-26646

Provider

Invitrogen Antibodies

Product name

Anti-Phospho-MEK1/MEK2 (Ser221) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-26646 detects MEK1 + MEK2 (phospho S222) from human, mouse, and rat samples. PA1-26646 is expected to cross react with chicken, chimpanzee, and Xenopus laevis due to sequence homology. PA1-26646 has been successfully used in Western blot procedures. The PA1-26646 immunogen is a synthetic phosphopeptide corresponding to a portion of human MEK 1 + 2 containing serine 222. The sequence is conserved in many species including mouse, rat, chimp, chicken, frog, and others.

Reactivity

Human, Mouse, Rat, Xenopus

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.5 mg/ml

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of Phospho-MEK1 + MEK2 pSer221 in extracts prepared from NIH-3T3 cells left untreated (lane 1) or treated (lanes 2-5) with PDGF. Samples were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4ºC and probed using a Phospho-MEK1 + MEK2 pSer221 polyclonal antibody (Product # PA1-26646).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-MEK1 + MEK2 pSer221 in mouse embryonic fibroblasts using a Phospho-MEK1 + MEK2 pSer221 polyclonal antibody (Product # PA1-26646) showing cell division. Blue represents chromosomes in anaphase of mitotic cell division. Green shows mitotic spindle expressing phosphorylated MEK1/2.