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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PPS076 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MEK1 Antibody
- Antibody type
- Polyclonal
- Description
- MEK1
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage, = -20degrees C is recommended. Product is stable at = -20degrees C for at least 1 year.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Phospho-MEK1 (T386) by Western Blot Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase, lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the MEK1 (T386) antibody. The immunolabeling of MEK1 is completely eliminated by lambda PPase.
