Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB9608 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9608, RRID:AB_1710045
- Product name
- MAP2K1 (phospho T292) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mechanisms of regulating the Raf kinase family.
Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.
Chong H, Vikis HG, Guan KL
Cellular signalling 2003 May;15(5):463-9
Cellular signalling 2003 May;15(5):463-9
Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Adams JP, Sweatt JD
Annual review of pharmacology and toxicology 2002;42:135-63
Annual review of pharmacology and toxicology 2002;42:135-63
Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.
Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG
Journal of biochemistry 1994 Aug;116(2):304-14
Journal of biochemistry 1994 Aug;116(2):304-14
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot of recombinant wild type and mutant MAP2K1 showing immunolabeling of the ~45k MAP2K1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MAP2K1 and Lanes 3 and 4 are mutant MAP2K1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.