PAB9609

antibody from Abnova Corporation

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9609

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9609, RRID:AB_1710048

Product name

MAP2K1 (phospho T386) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Mechanisms of regulating the Raf kinase family.

Chong H, Vikis HG, Guan KL
Cellular signalling 2003 May;15(5):463-9

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Molecular psychology: roles for the ERK MAP kinase cascade in memory.

Adams JP, Sweatt JD
Annual review of pharmacology and toxicology 2002;42:135-63

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Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.

Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG
Journal of biochemistry 1994 Aug;116(2):304-14

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of human T-47D cells showing specific immunolabeling of the ~45k MAP2K1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: lambda Ptase) The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the MAP2K1 (phospho T386) polyclonal antibody (Cat # PAB9609). The immunolabeling of MAP2K1 is completely eliminated by lambda Ptase.