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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB1953 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1953, RRID:AB_1678876
- Product name
- MAP2K1 monoclonal antibody, clone 263P15
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant MAP2K1.
- Isotype
- IgG
- Antibody clone number
- 263P15
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation.
Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions.
Structural organization of MAP-kinase signaling modules by scaffold proteins in yeast and mammals.
Slack-Davis JK, Eblen ST, Zecevic M, Boerner SA, Tarcsafalvi A, Diaz HB, Marshall MS, Weber MJ, Parsons JT, Catling AD
The Journal of cell biology 2003 Jul 21;162(2):281-91
The Journal of cell biology 2003 Jul 21;162(2):281-91
Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions.
Kolch W
The Biochemical journal 2000 Oct 15;351 Pt 2:289-305
The Biochemical journal 2000 Oct 15;351 Pt 2:289-305
Structural organization of MAP-kinase signaling modules by scaffold proteins in yeast and mammals.
Whitmarsh AJ, Davis RJ
Trends in biochemical sciences 1998 Dec;23(12):481-5
Trends in biochemical sciences 1998 Dec;23(12):481-5
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis using MAP2K1 monoclonal antibody, clone 263P15 (Cat # MAB1953), on human A-431 (1), mouse 3T3-L1 (2) and rat L6 (3) cell lysates. Antibody used at 1 ug/mL for two hours at room temperature.
