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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358157 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer217), (pSer221) antibody
- Antibody type
- Polyclonal
- Description
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer217, pSer221
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic stress.
MEK1 and MEK2, different regulators of the G1/S transition.
Dystroglycan, a scaffold for the ERK-MAP kinase cascade.
Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Naegele S, Morley SJ
The Journal of biological chemistry 2004 Oct 29;279(44):46023-34
The Journal of biological chemistry 2004 Oct 29;279(44):46023-34
MEK1 and MEK2, different regulators of the G1/S transition.
Ussar S, Voss T
The Journal of biological chemistry 2004 Oct 15;279(42):43861-9
The Journal of biological chemistry 2004 Oct 15;279(42):43861-9
Dystroglycan, a scaffold for the ERK-MAP kinase cascade.
Spence HJ, Dhillon AS, James M, Winder SJ
EMBO reports 2004 May;5(5):484-9
EMBO reports 2004 May;5(5):484-9
Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition.
Ohren JF, Chen H, Pavlovsky A, Whitehead C, Zhang E, Kuffa P, Yan C, McConnell P, Spessard C, Banotai C, Mueller WT, Delaney A, Omer C, Sebolt-Leopold J, Dudley DT, Leung IK, Flamme C, Warmus J, Kaufman M, Barrett S, Tecle H, Hasemann CA
Nature structural & molecular biology 2004 Dec;11(12):1192-7
Nature structural & molecular biology 2004 Dec;11(12):1192-7
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
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