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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
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- Product number
- NB500-145 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-145, RRID:AB_10002825
- Product name
- Rabbit Polyclonal MEK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~45k MEK 1 protein phosphorylated at Thr292 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms.
Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.
Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.
The MAP kinase cascade. Discovery of a new signal transduction pathway.
The MAP kinase cascade. Discovery of a new signal transduction pathway.
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin.
Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin.
Park SH, Zarrinpar A, Lim WA
Science (New York, N.Y.) 2003 Feb 14;299(5609):1061-4
Science (New York, N.Y.) 2003 Feb 14;299(5609):1061-4
Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Adams JP, Sweatt JD
Annual review of pharmacology and toxicology 2002;42:135-63
Annual review of pharmacology and toxicology 2002;42:135-63
Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.
Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG
Journal of biochemistry 1994 Aug;116(2):304-14
Journal of biochemistry 1994 Aug;116(2):304-14
Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.
Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG
Journal of biochemistry 1994 Aug;116(2):304-14
Journal of biochemistry 1994 Aug;116(2):304-14
The MAP kinase cascade. Discovery of a new signal transduction pathway.
Ahn NG
Molecular and cellular biochemistry 1993 Nov;127-128:201-9
Molecular and cellular biochemistry 1993 Nov;127-128:201-9
The MAP kinase cascade. Discovery of a new signal transduction pathway.
Ahn NG
Molecular and cellular biochemistry 1993 Nov;127-128:201-9
Molecular and cellular biochemistry 1993 Nov;127-128:201-9
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
Crews CM, Alessandrini A, Erikson RL
Science (New York, N.Y.) 1992 Oct 16;258(5081):478-80
Science (New York, N.Y.) 1992 Oct 16;258(5081):478-80
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
Crews CM, Alessandrini A, Erikson RL
Science (New York, N.Y.) 1992 Oct 16;258(5081):478-80
Science (New York, N.Y.) 1992 Oct 16;258(5081):478-80
Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin.
Ahn NG, Robbins DJ, Haycock JW, Seger R, Cobb MH, Krebs EG
Journal of neurochemistry 1992 Jul;59(1):147-56
Journal of neurochemistry 1992 Jul;59(1):147-56
Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin.
Ahn NG, Robbins DJ, Haycock JW, Seger R, Cobb MH, Krebs EG
Journal of neurochemistry 1992 Jul;59(1):147-56
Journal of neurochemistry 1992 Jul;59(1):147-56
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEK1 [p Thr292] Antibody [NB500-145] - Recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.
