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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-4630 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MEK1 (Thr292) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, non-human primate and rat based on 100% sequence homology. This antibody is specific for the ~45 kDa MEK 1 protein phosphorylated at Thr292 in Western blots of human brain extracts.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of recombinant WT and mutant MEK 1 immunolabeled with the Anti-Phospho-Thr292 MEK1 antibody. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of recombinant wild type MEK 1 (WT) and mutant MEK1 (Mut). Specific immunolabeling of a band at ~45 kDa corresponding to Phospho-MEK1 (Thr292) polyclonal antibody (Product # PA1-4630), which is shown in the second lane where MAP kinase was coexpressed.
