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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27693 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MEK1 Polyclonal Antibody (Product # PA5-27693). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. MEK1 Polyclonal Antibody (Product # PA5-27693) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MEK1 Polyclonal Antibody (Product # PA5-27693). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. MEK1 Polyclonal Antibody (Product # PA5-27693) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MEK1/MAP2K1 in paraformaldehyde-fixed HeLa cells using a MEK1/MAP2K1 polyclonal antibody (Product # PA5-27693) (Green) at a 1:200 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MEK1 was performed in paraffin-embedded rat liver tissue using MEK1 Polyclonal Antibody (Product # PA5-27693) at a dilution of 1:500.
