GTX25613

antibody from GeneTex

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX25613

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25613, RRID:AB_380596

Product name

MEK1 (phospho Ser298) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of NIH3T3 cells untreated (1) or treated with 50 ng/mL PDGF for 15 minutes (2-7) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either left untreated (1-5, 7) or treated with lambda phosphatase (6), blocked with a 4% BSA-TBST buffer for one hour at room temperature, then incubated with the MEK1 [pS298] antibody for two hours at room temperature in a 1% BSA-TBST, following prior incubation with: no peptide (1, 2, 6, 7), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4) or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and signals were detected. The data show that only the phosphopeptide corresponding to MEK1 [pS298] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

A549 cells plated on fibronectin, serum starved overnight then stimulated with serum for 1 hour. Green = MEK1 [pS298] Red = Actin Blue = Hoechst 33342