GTX25615

antibody from GeneTex

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25615

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25615, RRID:AB_380597

Product name

MEK1 (phospho Thr386) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of NIH3T3 cells untreated (1) or treated with 50ng/mL PDGF for 15 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 4% BSA-TBST buffer for one hour at room temperature, then incubated with the MEK1 [pT386] antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and signals were detected.The data show that only the phosphopeptide corresponding to MEK1 [pT386] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the upregulation of MEK1 [pT386] upon PDGF treatment in this cell system.