AF2079

antibody from R&D Systems

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2079

Provider

R&D Systems

Product name

Human/Mouse/Rat MEK1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat MEK1 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q02750


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat MEK1 by Western Blot. Western blot shows lysates of BT-20 human breast cancer cell line, U937 human histiocytic lymphoma cell line, DU145 human prostate carcinoma cell line, and mouse brain tissue. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse/Rat MEK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MEK1 (5 ng) was included. A specific band for MEK1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.