PA1-10034

antibody from Invitrogen Antibodies

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-10034

Provider

Invitrogen Antibodies

Product name

MEK1/MEK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Western Blot: Both reducing and non-reducing conditions, reducing conditions are recommended.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), H-1975 (Lane 2), HCT 116 (Lane 3), HT-29 (Lane 4), A-431 (Lane 5), A549 (Lane 6) MDA-MB-231 (Lane 7), U-87 MG (Lane 8), MCF7 (Lane 9), Raji (Lane 10) and NIH/3T3 (Lane 11). The blot was probed with Anti-MEK1/MEK2 Rabbit Polyclonal Antibody (Product # PA1-10034, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 45 kDa band corresponding to MEK1/MEK2 was detected across all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).