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LearnOAAB01768
antibody from Aviva Systems Biology
Targeting: RPS6KB1
p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- OAAB01768 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- RPS6KB1 Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (AP3289g) in human TNF alpha activated Hela cell lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alph
- Sample type
- Hela cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of RPS6KB1 (arrow) using rabbit polyclonal RPS6KB1 Antibody (S424) (RB11404). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or lysates transiently transfected transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies). )
- Sample type
- lysatestransiently transfected with the RPS6KB1 gene
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human breast carcinoma tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
