Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB5785 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB5785, RRID:AB_1579786
- Product name
- RPS6KB1 (phospho S424) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of RPS6KB1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis.
Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes.
Intracellular signaling of angiotensin II-induced p70 S6 kinase phosphorylation at Ser(411) in vascular smooth muscle cells. Possible requirement of epidermal growth factor receptor, Ras, extracellular signal-regulated kinase, and Akt.
Le XF, Hittelman WN, Liu J, McWatters A, Li C, Mills GB, Bast RC Jr
Oncogene 2003 Jan 30;22(4):484-97
Oncogene 2003 Jan 30;22(4):484-97
Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes.
Krause U, Bertrand L, Hue L
European journal of biochemistry / FEBS 2002 Aug;269(15):3751-9
European journal of biochemistry / FEBS 2002 Aug;269(15):3751-9
Intracellular signaling of angiotensin II-induced p70 S6 kinase phosphorylation at Ser(411) in vascular smooth muscle cells. Possible requirement of epidermal growth factor receptor, Ras, extracellular signal-regulated kinase, and Akt.
Eguchi S, Iwasaki H, Ueno H, Frank GD, Motley ED, Eguchi K, Marumo F, Hirata Y, Inagami T
The Journal of biological chemistry 1999 Dec 24;274(52):36843-51
The Journal of biological chemistry 1999 Dec 24;274(52):36843-51
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from 293 cells, untreated or treated with serum (10 %, 10 min), using RPS6KB1 (phospho S424) polyclonal antibody (Cat # PAB5785).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using RPS6KB1 (phospho S424) polyclonal antibody (Cat # PAB5785).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
