Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA502913 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502913, RRID:AB_11126077
- Product name
- RPS6KB1 (S6K) mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)
- Antibody type
- Monoclonal
- Description
- RPS6KB1 (S6K) mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RPS6KB1
- Isotype
- IgG
- Antibody clone number
- OTI1G4
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (RC217324, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-RPS6KB1 mouse monoclonal antibody (TA502913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1(RC217324).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502913)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502913)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502913)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC217324 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPS6KB1 antibody(TA502913), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-RPS6KB1 antibody(TA502913),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
