A01475-1
antibody from Boster Biological Technology
Targeting: RPS6KB1
p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A01475-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-p70 S6 Kinase (Ab-411) RPS6KB1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody for P23443 detection. Tested positive for WB IHC IF in Hu Ms Rt
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100ul
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Store at -20°C for long term preservation (recommended) . Store at 4°C for short term use.
Submitted references The VEGFR2/mTOR/S6K1 pathway involved in the angiogenic effects of roxarsone in vitro and in vivo.
Zhang M, Ding L, Zhou Z, Liu C, Wang C, Chen B, Chen X, Zhang Y
Toxicology 2022 Aug;478:153290
Toxicology 2022 Aug;478:153290
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of RPS6KB1 using anti-RPS6KB1 antibody (A01475-1).RPS6KB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RPS6KB1 Antibody (A01475-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image