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antibody from Novus Biologicals
Targeting: RPS6KB1
p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- NBP2-00754 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal p70 S6 Kinase/S6K Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S6K Antibody (1G4) [NBP2-00754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S6K (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S6K.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S6K Antibody (1G4) [NBP2-00754] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-S6K mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-S6K mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Human pancreas tissue using anti-S6K mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: S6K Antibody (1G4) [NBP2-00754] - Analysis of Jurkat cells, using anti-S6K antibody, (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: S6K Antibody (1G4) [NBP2-00754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-S6K antibody, and then analyzed by flow cytometry.
