BS7047

antibody from Bioworld Technology, Inc

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

BS7047

Provider

Bioworld Technology, Inc

Product name

Cleaved PARP 1 (D214) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to amino acids 180-214 of Human PARP-1.

Description

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of Cleaved-PARP-1 pAb at 1:1000 dilutionLane1:HEK293T whole cell lysate Lane2:HEK293T treated with PMA(1.54 mg/ml, 15min) whole cell lysate Lane3:THP-1 whole cell lysate Lane4:THP-1 treated with PMA(1.32 mg/ml, 5min) whole cell lysate