PA5-34802

antibody from Invitrogen Antibodies

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34802

Provider

Invitrogen Antibodies

Product name

PARP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, A549, PC-12, Rat2. Predicted reactivity: Mouse (84%), Rat (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Dissecting the molecular determinants of clinical PARP1 inhibitor selectivity for tankyrase1.

Ryan K, Bolaňos B, Smith M, Palde PB, Cuenca PD, VanArsdale TL, Niessen S, Zhang L, Behenna D, Ornelas MA, Tran KT, Kaiser S, Lum L, Stewart A, Gajiwala KS
The Journal of biological chemistry 2021 Jan-Jun;296:100251

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PARP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 Polyclonal Antibody (Product # PA5-34802) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PARP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 Polyclonal Antibody (Product # PA5-34802) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP assay analysis of PARP1 was performed in HeLa chromatin extract using 5 µg of either normal rabbit IgG or PARP1 Polyclonal Antibody (Product # PA5-34802). The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with Raji chromatin extract and 5 µg of either control rabbit IgG or PARP1 Polyclonal Antibody (Product # PA5-34802). The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PARP1 antibody [N1N2], N-term immunoprecipitates PARP1 protein in IP experiments. IP samples: HCT-116 whole cell extract. A. 30 µg HCT-116 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of PARP1 protein by 4 µg PARP1 antibody [N1N2], N-term (Product # PA5-34802). 5 % SDS-PAGE. The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N1N2], N-term (Product # PA5-34802) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Talazoparib binds to TNKS1 at a clinically relevant concentration in cultured cells. NCI-H1048 cell lysate was pretreated with indicated concentrations of talazoparib or olaparib (30 min) prior to the addition of talazoparib- or olaparib-biotin probes (30 min). Proteins bound to streptavidin were released and subjected to Western blot analysis for either PARP1 ( top ) or TNKS1 ( bottom ). Relative quantitation (Rel Quant) was determined using ImageJ and is displayed below the plots. PARP1, poly(ADP-ribosyl) polymerase 1; TNKS1, tankyrase1.