GTX61017

antibody from GeneTex

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Immunohistochemistry

Antibody data

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Validation data

Product number: GTX61017 - Provider product page
Provider: GeneTex
Proper citation: GeneTex Cat#GTX61017, RRID:AB_10620553
Product name: PARP (p25) antibody [E51]
Antibody type: Monoclonal
Antigen: Synthetic peptide corresponding to residues before the cleavage site of Human PARP.
Host: Rabbit
Isotype: IgG
Vial size: 100µl
Concentration: 0,377 mg/ml
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

PARP1 protein structure - GTX61017 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: A. Western blot analysis on Jurkat cell lysate using anti-PARP-1 Cleaved (p25) RabMAb (cat. #GTX61017) 1:50000 dilution. Cells were either (1) untreated or (2) treated with Camptothecin.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: WB analysis of untreated (1) and Camptothecin-treated WB analysis of Jurkat cell lysate usings using PARP (cleaved p25) antibody [E51] at a dilution of 1:1,000,000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: WB analysis of (1) RAW264.7 and (2) NIH/3T3 lysates (20 ?g per lane) using PARP (cleaved p25) antibody [E51] at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: WB analysis of lysates from (1) untreated and (2) camptothecin-treated Jurkat cells (10 ?g per lane) using PARP (cleaved p25) antibody [E51] at a dilution of 1:10000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: WB analysis of WB analysis of MCF7 cell lysate using PARP (cleaved p25) antibody [E51] at a dilution of 1:50,000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: WB analysis of lysates from Jurkat cells treated with vehicle control (0 ?M) and different concentrations of 15-Acetoxyscirpenol using PARP (cleaved p25) antibody [E51].
Validation comment: WB