Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 48-6668-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PARP1 (cleaved Asp214) Monoclonal Antibody (HLNC4), eFluor™ 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa.
- Antibody clone number
- HLNC4
- Concentration
- 5 µL/Test
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Intracellular staining of unstimulated (orange histogram) or staurosporine-stimulated (purple histogram) Jurkat cells with Anti-Human PARP1 (Cleaved) eFluor® 450 using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Total cells were used for analysis.