53-6668-42

antibody from Invitrogen Antibodies
Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL
Provider product page for 53-6668-42

Antibody data

Product number
53-6668-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
PARP1 (cleaved Asp214) Monoclonal Antibody (HLNC4), Alexa Fluor™ 488, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa. The HLNC4 antibody specifically recognizes the 85 kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116 kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH. Applications Reported: This HLNC4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This HLNC4 antibody has been pre-titrated and tested by flow cytometric analysis of staurosporine-stimulated Jurkat cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: intracellular Staining. Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Green dye
Isotype
IgG
Antibody clone number
HLNC4
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
PARP1 protein structure - 53-6668-42 shown in red.