Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 53-6668-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PARP1 (cleaved Asp214) Monoclonal Antibody (HLNC4), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa. The HLNC4 antibody specifically recognizes the 85 kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116 kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH. Applications Reported: This HLNC4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This HLNC4 antibody has been pre-titrated and tested by flow cytometric analysis of staurosporine-stimulated Jurkat cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: intracellular Staining. Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- HLNC4
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The role of proteases during apoptosis.
Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase.
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study.
Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase.
Poly(ADP-ribose) polymerase is a zinc metalloenzyme.
Patel T, Gores GJ, Kaufmann SH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1996 Apr;10(5):587-97
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1996 Apr;10(5):587-97
Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase.
Tewari M, Quan LT, O'Rourke K, Desnoyers S, Zeng Z, Beidler DR, Poirier GG, Salvesen GS, Dixit VM
Cell 1995 Jun 2;81(5):801-9
Cell 1995 Jun 2;81(5):801-9
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study.
Lamarre D, Talbot B, de Murcia G, Laplante C, Leduc Y, Mazen A, Poirier GG
Biochimica et biophysica acta 1988 Jul 13;950(2):147-60
Biochimica et biophysica acta 1988 Jul 13;950(2):147-60
Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase.
Lamarre D, Talbot B, Leduc Y, Muller S, Poirier G
Biochemistry and cell biology = Biochimie et biologie cellulaire 1986 Apr;64(4):368-76
Biochemistry and cell biology = Biochimie et biologie cellulaire 1986 Apr;64(4):368-76
Poly(ADP-ribose) polymerase is a zinc metalloenzyme.
Zahradka P, Ebisuzaki K
European journal of biochemistry 1984 Aug 1;142(3):503-9
European journal of biochemistry 1984 Aug 1;142(3):503-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining with Annexin V Apoptosis Detection Kit APC (Product # 88-8007-72) followed by intracellular staining of Jurkat cells stimulated with staurosporine for 2 hours (right) or left unstimulated (left) with Anti-Human PARP1 (Cleaved) Alexa Fluor® 488 (right) using Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). Total cells were used for analysis.
- Conjugate
- Green dye