14-6668-80

antibody from Invitrogen Antibodies
Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL
Provider product page for 14-6668-80
Western blot

Antibody data

Product number
14-6668-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-PARP1 (cleaved Asp214) Monoclonal Antibody (HLNC4), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa. The HLNC4 antibody specifically recognizes the 85 kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116 kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH. Applications Reported: This HLNC4 antibody has been reported for use in immunoblotting (WB). Applications Tested: This HLNC4 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
HLNC4
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
PARP1 protein structure - 14-6668-80 shown in red.