Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- LS-C745018 - Provider product page
- Provider
- LSBio
- Product name
- PARP1 Antibody (C-Terminus) LS-C745018
- Antibody type
- Polyclonal
- Description
- Affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load 5 ? per lane. Primary Antibody: Blot A: Anti-PARP1- n term; Blot B: Anti-PARP1- internal at 1?/mL for overnight at 4?. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4?. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): nonspecific ~ 40kDa in PARP1-AD only.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit Anti-PARP1 (internal) Antibody. Lane 1: Wild Type control lysates. Lane 2: Knock Out control lysates. Load: 5 µg per lane. Primary antibody: PARP1 (internal) antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~113kDa endogenous for PARP1. Other band(s): nonspecific ~ 40kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of recombinant PARP1 with Rabbit anti-PARP1 (internal) antibody. Lane 1: PARP1-autocatalytic domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (internal) antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP Gt-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~19 kDa for rPARP1 (internal) Other band(s): none.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit anti-PARP1 antibody. Lane 1: Molecular Weight ladder. Lane 2: OVCAR-8 Wild Type. Lane 3: PARP1-KO. Lane 4: PARP2-KO. Lane 5: PARP3-KO. Lane 6: PARP4-KO Lane 7: PARP5a-KO. Lane 8: PARP5b-KO. Lane 9: PARP6-KO. Lane 10: PARP7-KO. Lane 11: PARP8-KO. Lane 12: PARP9-KO. Lane 13: PARP10-KO. Lane 14: PARP12-KO. Lane 15: PARP13-KO. Lane 16: PARP14-KO. Lane 17: PARP16-KO. Load: 5.0 µg per lane. Primary antibody: PARP1 antibody at 1ug/mL overnight at 4°C. Secondary antibody: Goat anti-rabbit Peroxidase secondary antibody at 1:40,000 for 30 min at RT. Blocking Buffer: MB-073 for 30 min at RT. Predicted size: ~113kDa for PARP1. Observed nonspecific ~40kDa.