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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- 39562 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PARP-1 C-terminal Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PARP-1 C-terminal antibody tested by Western blot. HeLa nuclear extract (20 µg per lane) was probed with PARP-1 C-terminal antibody at a dilution of 1:6,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PARP-1 C-terminal antibody tested by Western blot. HeLa nuclear extract (20 µg per lane) was probed with PARP-1 C-terminal antibody at a dilution of 1:6,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PARP-1 C-terminal antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of PARP-1 C-terminal antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the BRCA2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
