PA1-38414

antibody from Invitrogen Antibodies

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-38414

Provider

Invitrogen Antibodies

Product name

PARP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, chicken, amphibian, mouse and rat based on sequence homology.

Concentration

0.2 mg/ml

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of PARP was achieved by transfecting HEK-293 with PARP specific siRNAs (Silencer® select Product # S1098, S1099). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the PARP knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PARP Polyclonal Antibody (Product # PA1-38414, 1ug/ml ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PARP.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PARP Polyclonal Antibody(Product # PA1-38414) and a 116kDa band corresponding to PARP was observed across all tested cell lines. Nuclear enriched extracts (30 µg lysate) of Jurkat (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Poly ADP-Ribose Polymerase using anti-Poly ADP-Ribose Polymerase Polyclonal Antibody (Product # PA5-32561) in Tonsil Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.