- PA5-27219 - Invitrogen Antibodies PARP1 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PARP1 using 30 µg of A) 293T and B) HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PARP1 polyclonal antibody (Product # PA5-27219) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PARP1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PARP1 Polyclonal Antibody (Product # PA5-27219) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PARP1 Polyclonal Antibody (Product # PA5-27219). Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 Polyclonal Antibody (Product # PA5-27219) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PARP1 Polyclonal Antibody (Product # PA5-27219). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP1 Polyclonal Antibody (Product # PA5-27219) diluted at 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PARP1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PARP1 Polyclonal Antibody (Product # PA5-27219) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PARP1 antibody detects PARP1 protein by western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated). B. 30 µg HCT116 whole cell lysate/extract (30 µM cisplatin treatment for 24hr).7.5% SDS-PAGE. PARP1 antibody PARP1 Polyclonal Antibody (Product # PA5-27219) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PARP1 was performed by separating 30 µg of untreated (–) and treated (+) HeLa whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PARP1 Polyclonal Antibody (Product # PA5-27219) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PARP1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PARP1 Polyclonal Antibody (Product # PA5-27219) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of PARP was performed using log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PARP Polyclonal Antibody (Product # PA5-27219) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization of PARP. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of PARP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 Polyclonal Antibody (Product # PA5-27219) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of PARP1 was performed in paraffin-embedded human oral carcinoma tissue using PARP1 Polyclonal Antibody (Product # PA5-27219) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Enrichment of endogenous PARP protein at specific gene loci using Anti-PARP Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-PARP Rabbit Polyclonal Antibody (Product # PA5-27219, 4 ug) on sheared chromatin from 2 million HeLa cells treated with Etoposide (50 uM for 6 hours) using the MAGnify ChIP System (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over IGFBP6, IGFBP7, GAPDH promoter and gene, SAT2 satellite repeats and SAT alpha. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Cross-linked ChIP was performed with Raji chromatin extract and 5 µg of either control rabbit IgG or PARP1 Polyclonal Antibody (Product # PA5-27219). The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.

PA5-27219