BML-SA249-0050

antibody from Enzo Life Sciences

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BML-SA249-0050

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#BML-SA249-0050, RRID:AB_11180099

Product name

Poly(ADP-ribose) polymerase, mAb (C-2-10) (affinity purified)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

Affinity purified. Liquid. In PBS containing 0.02% sodium azide.

Isotype

IgG

Antibody clone number

C-2-10

References

Submitted references

PRIMA-1(MET) induces death in soft-tissue sarcomas cell independent of p53.

Grellety T, Laroche-Clary A, Chaire V, Lagarde P, Chibon F, Neuville A, Italiano A
BMC cancer 2015 Oct 13;15:684

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Alzheimer's disease-associated polymorphisms in human OGG1 alter catalytic activity and sensitize cells to DNA damage.

Jacob KD, Noren Hooten N, Tadokoro T, Lohani A, Barnes J, Evans MK
Free radical biology & medicine 2013 Oct;63:115-25

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Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis.

Leprêtre C, Scovassi AI, Shah GM, Torriglia A
The international journal of biochemistry & cell biology 2009 May;41(5):1046-54

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CCCTC-binding factor activates PARP-1 affecting DNA methylation machinery.

Guastafierro T, Cecchinelli B, Zampieri M, Reale A, Riggio G, Sthandier O, Zupi G, Calabrese L, Caiafa P
The Journal of biological chemistry 2008 Aug 8;283(32):21873-80

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Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction.

Cipriani G, Rapizzi E, Vannacci A, Rizzuto R, Moroni F, Chiarugi A
The Journal of biological chemistry 2005 Apr 29;280(17):17227-34

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Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio.

Javelaud D, Besancon F
The Journal of biological chemistry 2002 Oct 4;277(40):37949-54

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Characterization of the necrotic cleavage of poly(ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases.

Gobeil S, Boucher CC, Nadeau D, Poirier GG
Cell death and differentiation 2001 Jun;8(6):588-94

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Selective loss of poly(ADP-ribose) and the 85-kDa fragment of poly(ADP-ribose) polymerase in nucleoli during alkylation-induced apoptosis of HeLa cells.

Alvarez-Gonzalez R, Spring H, Müller M, Bürkle A
The Journal of biological chemistry 1999 Nov 5;274(45):32122-6

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Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis.

Datta R, Kojima H, Banach D, Bump NJ, Talanian RV, Alnemri ES, Weichselbaum RR, Wong WW, Kufe DW
The Journal of biological chemistry 1997 Jan 17;272(3):1965-9

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Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase.

Duriez PJ, Desnoyers S, Hoflack JC, Shah GM, Morelle B, Bourassa S, Poirier GG, Talbot B
Biochimica et biophysica acta 1997 Feb 11;1334(1):65-72

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FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis.

Chinnaiyan AM, Tepper CG, Seldin MF, O'Rourke K, Kischkel FC, Hellbardt S, Krammer PH, Peter ME, Dixit VM
The Journal of biological chemistry 1996 Mar 1;271(9):4961-5

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Different cleavage pattern for poly(ADP-ribose) polymerase during necrosis and apoptosis in HL-60 cells.

Shah GM, Shah RG, Poirier GG
Biochemical and biophysical research communications 1996 Dec 24;229(3):838-44

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Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution.

Lazebnik YA, Takahashi A, Moir RD, Goldman RD, Poirier GG, Kaufmann SH, Earnshaw WC
Proceedings of the National Academy of Sciences of the United States of America 1995 Sep 26;92(20):9042-6

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Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE.

Lazebnik YA, Kaufmann SH, Desnoyers S, Poirier GG, Earnshaw WC
Nature 1994 Sep 22;371(6495):346-7

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Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis.

Kaufmann SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirier GG
Cancer research 1993 Sep 1;53(17):3976-85

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Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study.

Lamarre D, Talbot B, de Murcia G, Laplante C, Leduc Y, Mazen A, Poirier GG
Biochimica et biophysica acta 1988 Jul 13;950(2):147-60

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101) and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The nitrocellulose membrane was probed with purified clone C-2-10 mouse monoclonal anti-PARP (Prod. No. BML-SA249) at 1 µg/ml. Secondary antibody was GAM-AP (1/2000) and the nitrocellulose membrane was developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.