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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-SA250-0050 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA250-0050, RRID:AB_2052271
- Product name
- Poly(ADP-ribose) polymerase, mAb (C-2-10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- Liquid. Mouse ascites containing 0.02% sodium azide.
- Isotype
- IgG
- Antibody clone number
- C-2-10
- Vial size
- 50 µl
Submitted references Novel p53 target genes secreted by the liver are involved in non-cell-autonomous regulation.
FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression.
Charni M, Molchadsky A, Goldstein I, Solomon H, Tal P, Goldfinger N, Yang P, Porat Z, Lozano G, Rotter V
Cell death and differentiation 2016 Mar;23(3):509-20
Cell death and differentiation 2016 Mar;23(3):509-20
FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression.
Xu HD, Cho SC, Bang MA, Bae CS, Choi Y, Li YC, Lim SK, Shim J, Park DH
International journal of oncology 2015;46(6):2309-16
International journal of oncology 2015;46(6):2309-16
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101] and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The membrane was probed with C-2-10 mouse monoclonal antibody (Prod. No. BML-SA250) at 1:1000 dilution, secondary antibody was GAMAP (1:2000) and developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.
