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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX628836 - Provider product page
- Provider
- GeneTex
- Product name
- PARP antibody [GT6212]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat
- Host
- Mouse
Submitted references A high-throughput pipeline for validation of antibodies.
Regulation of ABCC6 trafficking and stability by a conserved C-terminal PDZ-like sequence.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Regulation of ABCC6 trafficking and stability by a conserved C-terminal PDZ-like sequence.
Xue P, Crum CM, Thibodeau PH
PloS one 2014;9(5):e97360
PloS one 2014;9(5):e97360
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extractE. 30 ?g A375 whole cell lysate/extract7.5 % SDS-PAGEPARP1 antibody [GT6212] (GTX628836) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis.A. 30 ?g HCT116 whole cell lysate/extract (untreated)B. 30 ?g HCT116 whole cell lysate/extract (30?M Cisplatin treatment for 24hr)7.5 % SDS-PAGEPARP1 antibody [GT6212] (GTX628836) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PARP antibody [GT6212] detects PARP protein at nucleus in human Hep G2 by immunohistochemical analysis. Sample: Paraffin-embedded human Hep G2. PARP antibody [GT6212] (GTX628836) diluted at 1:250.
